Determine the field gradient required to separate two polypeptides (D = 5 x 10 -6 cm 2

Question:

Determine the field gradient required to separate two polypeptides (D =  5 x 10-6 cm2/s), with respective pI values of 4.2 and 4.8, using IEF with a linear field gradient of 0.3/cm. Use a standard criteria for IEF that peak-to-peak separation must be three times the average peak width.

Fantastic news! We've Found the answer you've been seeking!

Step by Step Answer:

Related Book For  book-img-for-question

Separation Process Principles Chemical And Biochemical Principles

ISBN: 9780470481837

3rd Edition

Authors: By J. D. Seader, Ernest J. Henley, D. Keith Roper

Question Posted: