Check the assumptions for the randomized block ANOVA of Exercise 14.25.

Data from Exercise 14.25

Refer to the Journal of Bacteriology (July 2002) study of the sensitivity of bacteria genes to light, Exercise 8.49 (p. 406). Recall that scientists isolated 103 genes of the bacterium responsible for photosynthesis and respiration. Each gene was grown to midexponential phase in a growth incubator in “full light,” then exposed to three alternative light/dark conditions: “full dark” (lights extinguished for 24 hours), “transient light” (lights turned back on for 90 minutes), and “transient dark” (lights turned back off for an additional 90 minutes). At the end of each light/dark condition, the standardized growth measurement was determined for each of the 103 genes. The complete data set is saved in the GENEDARK file. Assume that the goal of the experiment is to compare the mean standardized growth measurements for the three light/dark conditions.

Data from Exercise 8.49

Synechocystis, a type of cyanobacterium that can grow and survive in a wide range of conditions, is used by scientists to model DNA behavior. In the Journal of Bacteriology (July 2002), scientists isolated genes of the bacterium responsible for photosynthesis and respiration and investigated the sensitivity of the genes to light. Each gene sample was grown to midexponential phase in a growth incubator in “full light.” The lights were extinguished and growth measured after 24 hours in the dark (“full dark”). The lights were then turned back on for 90 minutes (“transient light”) followed immediately by an additional 90 minutes in the dark (“transient dark”). Standardized growth measurements in each light/dark condition were obtained for 103 genes. The complete data set is saved in the GENEDARK file. Data for the first 10 genes are shown in the accompanying table.

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GENEDARK (First 10 Observations Shown) Gene ID Full-Dark Tr-Light Tr-Dark SLR2067 -0.00562 1.40989 -1.28569 SLR1986 -0.68372 1.83097 -0.68723 SSR3383 -0.25468 -0.79794 -0.39719 SLL0928 -0.18712 -1.20901 -1.18618 SLRO335 -0.20620 1.71404 -0.73029 SLR1459 -0.53477 2.14156 -0.33174 SLL1326 -0.06291 1.03623 0.30392 SLR1329 -0.85178 -0.21490 0.44545 SLL1327 0.63588 1.42608 --0.13664 SLL1325 -0.69866 1.93104 -0.24820