Diafiltration is used to purify 40 L of dilute protein solution produced in a pilot plant. The

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Diafiltration is used to purify 40 L of dilute protein solution produced in a pilot plant. The solution has 0.8 percent protein and 3.2 percent salts, and the protein/salt ratio should be 50:1 after diafiltration.

(a) If pure water is added intermittently, 40 L at a time, what is the total amount of water needed? 

(b) How much water would be needed with continuous addition and constant retentate volume?

(c) If a subsequent UF step raises the protein concentration to 5 percent, what is the final salt concentration?

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Unit Operations Of Chemical Engineering

ISBN: 9780072848236

7th Edition

Authors: Warren McCabe, Julian Smith, Peter Harriott

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