A chromatographic procedure separates 4.0 mg of unknown mixture on a column with a length of 40
Question:
(a) What size column would you use to separate 100 mg of the same mixture?
(b) If the flow is 0.22 mL/min on the small column, what volume flow rate should be used on the large column?
(c) If mobile phase occupies 35% of the column volume, calculate the linear flow rate for the small column and the large column.
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a b c Large load Small load 100 mg Large column diameter 40 mg 085 cm diameter Large column radius ...View the full answer
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