A membrane glycoprotein-synthesizing system was devised that contained endoplasmic reticulum membranes and all the necessary ingredients for

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A membrane glycoprotein-synthesizing system was devised that contained endoplasmic reticulum membranes and all the necessary ingredients for the in vitro synthesis of a protein. The complete synthesis of the protein molecule required 40 minutes. When the detergent Triton X-100 was added within a few minutes after the initiation of polypeptide synthesis, the resulting protein was devoid of glycosyl groups.
(a) Explain the action of the detergent.
(b) Would the protein have been glycosylated if the detergent had been added 35 minutes after initiation of protein synthesis?
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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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