A mixture of 14 compounds was subjected to a reversedphase gradient separation going from 5% to 100% acetonitrile with a gradient time of 60 min. The sample was injected at t = dwell time. All peaks were eluted between 22 and 41 min.
(a) Is the mixture more suitable for isocratic or gradient elution?
(b) If the next run is a gradient, select the starting and ending %acetonitrile and the gradient time.