Question: Biochemical connections If you wanted to make a HEPES buffer at pH 8.3, and you had both HEPES acid and HEPES base avail-able, which would

Biochemical connections If you wanted to make a HEPES buffer at pH 8.3, and you had both HEPES acid and HEPES base avail-able, which would you start with, and why?

Step by Step Solution

★★★★★

3.39 Rating (158 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

It would be more effective to start with the HEPES base Y... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

481-M-S-B-C (40).docx

120 KBs Word File

Students Have Also Explored These Related Biochemistry Questions!

If you wanted to make a more compact ac generator (operating at the same frequency) by reducing the area of the coils, how would could you compensate by changing its other physical characteristics in...

If you wanted to make a scale model of air at 0.0 C and 1.00 atm, using ping-pong balls (diameter, 3.75 cm) to represent the N2 molecules (diameter, 0.30 nm), (a) How far apart on average should the...

If you wanted to have $500,000 one year from today and desired to earn a 10 percent return, what amount would you need to invest today? Which amount has more value, the amount today or the $500,000 a...

What would be the pH of the solution in Question 43 if you were to add 3.0 mL more of 1 M HCl? Question 43 If you have 100 mL of a 0.10 M TRIS buffer at pH 8.3 (Table 2.8) and you add 3.0 mL of 1 M...

If you have 100 mL of a 0.10 M TRIS buffer at pH 8.3 (Table 2.8) and you add 3.0 mL of 1 M HCl, what will be the new pH? Table 2.8 Acid and Base Form of Some Useful Biochemical Buffers Name Acid Form...

What would be the pH of the solution described in Question 41? Question 41 If you mix equal volumes of 0.1 M HCl and 0.20 M TRIS (free amine form; see Table 2.8), is the resulting solution a buffer?...

Which of the buffers shown in Table 2.8 would you choose to make a buffer with a pH of 7.3? Explain why. Table 2.8 Acid and Base Form of Some Useful Biochemical Buffers Name Acid Form...

16.The acid known as HEPES has a pKa of 7.55 and the known substance TRIS has a pKa of 8.3. Which of the following statements is true? a. HEPES would have a better capacity to buffer at a pH 8. b....

Based on information below: A. Internal Analysis 1. What is the analysis of the company's resource and capability at the corporate level? 2. Strategic Business Unit Portfolio ? What is the primary...

Biochemical coNNectioNs How is the importance of folic acid related to chemotherapy?

Assume that Clark, Eric, and Emily form another entity, DNA Solutions Inc. that will operate as a partnership. Emily contributes $50,000 in cash for her 10% interest in the entity, Eric contributes...

A filter is designed with the following specifications. The anthracite and sand have density of 1700 and 2650 kg/m, respectively, and the design temperature is 10C. For a given sample (A, B, C, D, or...

A cash discount calls for a reduction in price if payment cannot be made within a specified time period. Group of answer choices false true

(a) A consumer organisation is testing two different brands of battery. A sample of 15 of brand A shows an average useful life of 410 hours with a standard deviation of 20 hours. For brand B, a...

Biochemical connections Suggest a suitable buffer range for each of the following substances: (a) Lactic acid (pKa = 3.86) and its sodium salt (b) Acetic acid (pKa = 4.76) and its sodium salt (c)...

Biochemical connections If you wanted to make a HEPES buffer at pH 8.3, and you had both HEPES acid and HEPES base avail-able, which would you start with, and why?

Which would you think would be a stronger interaction and why: an interaction between a sodium ion and the partial negative charge on the oxygen in ethanol (CH3CH2OH), or the interaction between two...

Integrative-Risk and Valuation. Hamlin Steel Company Wishes to determine the value of Craft Foundry, a firm that is considering acquiring for cash. Hamlin wishes to determine the applicable discount...

otto enterprises has a 15 year bond issue outstanding with a coupon of 8%. the bond is currently priced at $923.60 and has a par value of $1,000. interest is paid semiannually. what is the yield to...

As CFO of Portobello Scuba Diving Inc., you are asked to look into the possibility of implementing a system to expedite cash receipts from clients. Portobello receives check remittances totaling $...