Question: Design a radiotracer experiment that would allow you to determine which proportion of glucose catabolism in a given tissue preparation occurs through the pentose phosphate
Design a radiotracer experiment that would allow you to determine which proportion of glucose catabolism in a given tissue preparation occurs through the pentose phosphate pathway and which proportion occurs through glycolysis and the citric acid cycle. Assume that you can synthesize glucose labeled with 14C in any desired position or combination of positions. Assume also that you can trap CO2 after administration of labeled glucose and determine its radioactivity.
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