Determine the amino acid sequence of a polypeptide from the following results:
a. Complete hydrolysis of the peptide yields the following amino acids: Ala, Arg, Gly, 2 Lys, Met, Phe, Pro, 2 Ser, Tyr, Val.
b. Treatment with Edman's reagent gives PTH-Val.
c. Carboxypeptidase A releases Ala.
d. Treatment with cyanogen bromide yields the following two peptides:
1. Ala, 2 Lys, Phe, Pro, Ser, Tyr
2. Arg, Gly, Met, Ser, Val
e. Treatment with chymotrypsin yields the following three peptides:
1. 2 Lys, Phe, Pro
2. Arg, Gly, Met, Ser, Tyr, Val
3. Ala, Ser
f. Treatment with trypsin yields the following three peptides:
1. Gly, Lys, Met, Tyr
2. Ala, Lys, Phe, Pro, Ser
3. Arg, Ser, Val