Differential centrifugation, the lying (bursting) of E. coli cells has produced a dilute suspension of inclusion bodies, hard insoluble aggregates of a desired protein, unlysed cells, and unwanted dissolved proteins. For purposes of this problem all may be considered as spheres can these materials be effectively separated by centrifugation? Explain.

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Inclusion bodies Proteins Cells Mass or equivalent Density (g/ml) 1.89 × 10-12 g 2.32 x 10-15 g 50 kilodaltons 1.07 1.3 1.3