Differential centrifugation, the lying (bursting) of E. coli cells has produced a dilute suspension of inclusion bodies, hard insoluble aggregates of a desired protein, unlysed cells, and unwanted dissolved proteins. For purposes of this problem all may be considered as spheres can these materials be effectively separated by centrifugation? Explain.

Inclusion bodies Proteins Cells Mass or equivalent Density (g/ml) 1.89 10-12 g 2.32 x 10-15 g 50 kilodaltons 1.07 1.3 1.3