How could you detect a colony containing a cloned gene if you did not know the gene

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How could you detect a colony containing a cloned gene if you did not know the gene sequence but had available purified protein encoded by the gene?
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Brock Biology of Microorganisms

ISBN: 978-0321897398

14th edition

Authors: Michael Madigan, John Martinko, Kelly Bender, Daniel Buckley

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