Refer to Exercise 18.30.
In Exercise 18.30
The following data are from Gennings, Chinchilli, and Carter, (1989). An in vitro toxicity study of isolated hepatocyte suspensions was conducted to study the impact of combining carbon tetrachloride (CCl4) and chloroform (CHCl3) on the toxicity of cells. Cell toxicity was measured by the amount of­lactic dehydrogenase (LDH) enzyme leakage. The study involved randomly assigning four flasks to each of the 16 treatments obtained by combining four levels of CCl4 (0, 1.0, 2.5, and 5.0 mM) with four levels of CHCl3 (0, 5, 10, and 25 mM). The percentage of LDH leakage from the cells in each of the 64 flasks was measured just prior to applying the treatment to the flasks and at .01, .25, .5, 1, 2, and 3 hours after applying the treatment. The percentages of LDH leakage are given in the following table.

a. Run a repeated measures analysis of variance, and determine if there are significant interaction and/or main effects due to CCl4 and CHCl3. Is there a significant time effect?
b. Do the conditions necessary for using a split-plot analysis of repeated measures data appear to be valid?

Transcribed Image Text:

3,0--10 11 2 1 22 23 .4 .4 13 13 18 21 32 27 S6 55 20 31 31 32 36 34 72 48 71 73 33 32 40 29 66 57 09-10-10 15 22 22 37 36 12 12 .20 21 꼬 27 .u A3 21 29 27 30 34 34 54 A9 60 62 28 27 37 27 53 41 rea-.5-08 07 13 18 16 24 24 10 09 16 18 27 21 22 27 19 23 23 25 26 23 29 25 50 44 22 21 29 16 31 .20 ine-25-08 06 13 07 17 15 17 16-10 08 14 15 24 16 15 15 18 21 21 33 19 21 15 45 42 .20 17 25-12 23-12 Tim-" 08 08 07 06 07 105 07 06 08 09-10 08 07 06 06 05 08 08 07 09 09 05 04 08-10-10 08 09 06 04 o| 07 D6 05 06 06 07 05 05 06 05 05 05 06 06 05 05 06 06 06 06 04 05 06 06 05 05 06 05 105屆 005511 25 25 0 0 5500 25 25 0 0 5510 25 25 0 0 5 5 10 25 25 00000000111111115555555555555555 46 34 70 51 21 A6 21 63 29 67 72 34 23 70 .30 58 .30 67 45 76 79 41 28 47 31 ibb 67 |-10 13 21 32 66 52 19 42 30 .50 35 53 75 33 28 66 35 49 47 67 46 73 73 36 32 39 36 66 58 1.8-10-12 14 .20 A6 29 65 A8 10 16 41 29 57 30 58 42 64 23 62 30 43 43 65 35 78 57 30 27 33 31 60 Al .5-08 09-12 36 27 51 39 09 37 22 61 .30 39 40 56 21 57 28 33 37 59 31 77 27 22 26 27 55 31 09 10 14 16 20 24 25 33 13 15 18 22 25 26 23 28 19 19 22 24 28 23 22 24 52 60 21 21 24 23 21 23 co nan anc 08 08 06 11 06 08 07 11 D6 08 Los 10 06 11 07 08 06-10 07 07 £6 08届09 D6 08 05 09 04-11 07 08 005500 25 25 0 0 5 5 0 0 25 25 0 5 511 25 25 0 0 5 5 1 25 25 5 5 5 5 5 5 5 5 o 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 5 5 5 5 5 5 5 5