Retention factors for three solutes separated on a C8 nonpolar stationary phase are listed in the table
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Retention factor Analyte Acetophenone Salicylic acid Nicotine pH 3 4.21 2.97 0.00 pH 5 4.28 0.65 0.13 pH 7 4.37 0.62 3.11 CO,H он CH3 Salicylic acic Nicotine pK1 3.15 pK2 7.85 pK 2.97
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Acetophenone is neutral at all pH values Its retention is nearly unaffected by pH For salic...View the full answer
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