An article in Journal of Chemical Technology and Biotechnology [“A Study of Antifungal Antibiotic Production by Thermomonospora sp MTCC 3340 Using Full Factorial Design” (2003, Vol. 78, pp. 605-610)] considered the effects of several factors on antifungal activities. The antifungal yield was expressed as Nystatin international units per cm³. The results from carbon source concentration (glucose) and incubation temperature factors follow. See Table E14-1.

(a) State the hypotheses of interest.

(b) Test your hypotheses with α = 0.5.

(c) Analyze the residuals and plot the residuals versus the predicted yield.

(d) Using Fisher’s LSD method, compare the means of antifungal activity for the different carbon source concentrations.