Shown below is a schematic diagram of the E. coli leader peptidase (Lep), which has several basic

Question:

Shown below is a schematic diagram of the E. coli leader peptidase (Lep), which has several basic amino acids in a cytoplasmic loop. Propose a mutant of Lep that would be a test of the “inside positive” rule for the orientation of proteins in membranes.

PERIPLASM MEMBRANE CYTOPLASM N Jagua +++ wt LEP C