The objective of a study by Abrahamsson et al. (A-22) was to investigate the anti-thrombotic effects of an inhibitor of the plasminogen activator inhibitor-1 (PAI-1) in rats given endotoxin. Experimental subjects were male Sprague–Dawley rats weighing between 300 and 400 grams. Among the data collected were the following measurements on PAI-1 activity and the lung 125I-concentration in anesthetized rats given three drugs:

Drugs (U/ml) (% of Ref. Sample)
Endotoxin 127 158 175 154 161 118 137 77 219 172 260 277 203 216 195 169 414 272 244 192 Endotoxin PRAP-1 low dose 107 49 103 28 248 187 164 109 176 96 230 126 184 148 276 17 201 97 158 86 Endotoxin PRAP-1 high dose 132 86 130 24 75 17 140 41 166 114 194 110 121 26 111 53 208 71 211 90 Source: Dr. Tommy Abrahamsson. Used with permission.
26. Pearse and Sylvester (A-23) conducted a study to determine the separate contributions of ischemia and extracorporeal perfusion to vascular injury occurring in isolated sheep lungs and to determine the oxygen dependence of this injury. Lungs were subjected to ischemia alone, extracorporeal perfusion alone, and both ischemia and extracorporeal perfusion. Among the data collected were the following observations on change in pulmonary arterial pressure (mm Hg)
and pulmonary vascular permeability assessed by estimation of the reflection coefficient for albumin in perfused lungs with and without preceding ischemia:

Ischemic–Perfused Lungs Perfused Lungs Change in Change in Pulmonary Reflection Pulmonary Reflection Pressure Coefficient Pressure Coefficient 8.0 0.220 34.0 0.693 3.0 0.560 31.0 0.470 10.0 0.550 4.0 0.651 23.0 0.806 48.0 0.999 15.0 0.472 32.0 0.719 43.0 0.759 27.0 0.902 18.0 0.489 25.0 0.736 27.0 0.546 25.0 0.718 13.0 0.548 0.0 0.467