Effects of temperature on ethanol production. A lowcost and highly productive bio-fuel production method is high-temperature fermentation. However, heat stress can inhibit the amount of ethanol produced during the process.

In Engineering Life Sciences (Mar. 2013), biochemical engineers carried out a series of experiments to assess the effect of temperature on ethanol production during fermentation.

The maximum inhibitory concentration of ethanol (grams per liter) was measured at four different temperatures (30°, 35°, 40°, and 45° Celsius). The experiment was replicated 3 times, with the data shown in the table. (Note: The data are simulated based on information provided in the journal article.) Do the data indicate that high temperatures inhibit mean concentration of ethanol? Test using a = .10.

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Temperature 30 35 40 45 103.3 101.7 97.2 55.0 103.4 102.0 96.9 56.4 101.0 101.1 96.2 54.9