Paper chromatography was used to separate a mixture of amino acids. The mixture was run in two

Question:

Paper chromatography was used to separate a mixture of amino acids. The mixture was run in two dimensions using two different solvents. The chromatogram obtained is shown below.

a. Explain briefly how the chromatography was carried out.

b. Which amino acids were inseparable using solvent 1? (Give just the corresponding letters.)

c. How could the amino acids be located?

d. Give the R_f value for amino acid C in each solvent.

e. Which amino acids would have been inseparable by solvent 2 alone?

f. In another experiment, a mixture of radioactively labelled amino acids was separated using paper chromatography. The results obtained are shown below.