A. How many different DNA fragments would you expect to obtain if you cleaved human genomic DNA
Question:
A. How many different DNA fragments would you expect to obtain if you cleaved human genomic DNA with HaeIII? How many fragments would you expect with EcoRI?
B. Human genomic libraries used for DNA sequencing are often made from fragments obtained by cleaving human DNA with HaeIII in such a way that the DNA is only partially digested; that is, not all the possible HaeIII sites have been cleaved. What is a possible reason for doing this?
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A To answer this question we need to consider the recognition sequences for the restriction enzymes HaeIII and EcoRI HaeIII The recognition sequence f...View the full answer
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Related Book For 
Essential Cell Biology
ISBN: 9780393680362
5th Edition
Authors: Bruce Alberts, Karen Hopkin, Alexander Johnson, David Morgan, Martin Raff, Keith Roberts, Peter Walter
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