A molecule of double-stranded DNA was cleaved with restriction enzymes, and the resulting products were separated by gel electrophoresis (Figure Q10–11). You do not know if the molecule is linear DNA or a DNA circle. DNA fragments of known sizes were electrophoresed on the same gel for use as size markers (left lane). The size of the DNA markers is given in kilobase pairs (kb), where 1 kb = 1000 nucleotide pairs. Using the size markers as a guide, estimate the length of each restriction fragment obtained. From this information, construct a map of the original DNA molecule indicating the relative positions of all the restriction enzyme cleavage sites.

Figure Q10–11

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nucleotide pairs (kb) 81 5 45 3.51 size Hindill + markers EcoRI Hindill EcoRI | Figure Q10-11 | | |