Jane has isolated a mutant strain of yeast that forms red colonies instead of the normal white

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Jane has isolated a mutant strain of yeast that forms red colonies instead of the normal white when grown on a plate. To determine the mutant gene, she decides to use functional complementation with a DNA library containing a lysine selection marker. lu addition to the unknown gene mutation, the yeast are lacking the gene required to synthesize the amino acids leucine and lysine. What media will Jane grow her yeast on to ensure that they have acquired the library plasmids? How will she know when a library plasmid has complemented her yeast mutation?

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Molecular Cell Biology

ISBN: 978-1429234139

7th edition

Authors: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott

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