To understand how a signaling pathway works, it often is useful to isolate the cell-surface receptor and

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To understand how a signaling pathway works, it often is useful to isolate the cell-surface receptor and to measure the activity of downstream effector proteins under different conditions. How could you use affinity chromatography to isolate a cell-surface receptor? With what technique could you measure the amount of activated G protein {the GTP-bound form) in ligand-stimulated cells? Describe the approach you would take.

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Molecular Cell Biology

ISBN: 978-1429234139

7th edition

Authors: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott

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