To understand how a signaling pathway works, it often is useful to isolate the cell-surface receptor and
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To understand how a signaling pathway works, it often is useful to isolate the cell-surface receptor and to measure the activity of downstream effector proteins under different conditions. How could you use affinity chromatography to isolate a cell-surface receptor? With what technique could you measure the amount of activated G protein {the GTP-bound form) in ligand-stimulated cells? Describe the approach you would take.
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Related Book For
Molecular Cell Biology
ISBN: 978-1429234139
7th edition
Authors: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott
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