In the MichaelisMenten mechanism, it is assumed that the formation of product from the enzymesubstrate complex is

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In the Michaelis€“Menten mechanism, it is assumed that the formation of product from the enzyme€“substrate complex is irreversible. However, consider the following modified version in which the product formation step is reversible:

k, E + Sa E + P kz ES k1

Derive the expression for the Michaelis constant for this mechanism in the limit where

[S]0 >> [E]0.    

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Physical Chemistry

ISBN: 978-0321812001

3rd edition

Authors: Thomas Engel, Philip Reid

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