One of the procedures for cloning foreign DNA segments takes advantage of restriction endonucleases such as HindIII that produce complementary singlestranded ends. These enzymes produce identical complementary ends on cleaved foreign DNAs and on the vector DNAs into which the foreign DNAs are inserted.
Assume that you have inserted your favorite gene into the HindIII site in the polycloning region of the Bluescript cloning vector with DNA ligase, have amplified the plasmid containing your gene in E. coli, and have isolated a large quantity of gene/Bluescript DNA. How could you excise your favorite gene from the Bluescript vector?