A particular gene is inserted into the phage lambda chromosome and is shown to contain three introns.
Question:
A particular gene is inserted into the phage lambda chromosome and is shown to contain three introns.
(a) The primary transcript of this gene is purified from isolated nuclei. When this primary transcript is hybridized under R-loop conditions with the recombinant lambda chromosome carrying the gene, what will the R-loop structure(s) look like? Label your diagram.
(b) The mRNA produced from the primary transcript of this gene is then isolated from cytoplasmic polyribosomes and similarly examined by the R-loop hybridization procedure using the recombinant lambda chromosome carrying the gene. Diagram what the R-loop structure(s) will look like when the cytoplasmic mRNA is used. Again, label the components of your diagram.
Step by Step Answer:
Principles of Genetics
ISBN: 978-1119142287
7th edition
Authors: D. Peter Snustad, Michael J. Simmons