The composite transposon Tn5 consists of two IS50 elements, one on either side of a group of

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The composite transposon Tn5 consists of two IS50 elements, one on either side of a group of three genes for antibiotic resistance. The entire unit IS50L kanr bler strr IS50R can transpose to a new location in the E. coli chromosome. 

However, of the two IS50 elements in this transposon, only IS50R produces the catalytically active transposase. Would you expect IS50R to be able to be excised from the Tn5 composite transposon and insert elsewhere in the chromosome? Would you expect IS50L to be able to do this?

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Principles of Genetics

ISBN: 978-1119142287

7th edition

Authors: D. Peter Snustad, Michael J. Simmons

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