Z. Ivics, Z. Izsvk, and P. B. Hackett have resurrected a nonmobile member of the Tc1/marinerfamily of
Question:
Z. Ivics, Z. Izsvák, and P. B. Hackett have “resurrected” a nonmobile member of the Tc1/marinerfamily of trans-posable elements isolated from the DNA of salmon. These researchers altered 12 codons within the coding sequence of the transposase gene of the salmon element to restore the catalytic function of its transposase. The altered element, called Sleeping Beauty, is being tested as an agent for the genetic transformation of vertebrates such as mice and zebra fish (and possibly humans). Suppose that you have a bacterial plasmid containing the gene for green fluorescent protein (gfp) inserted between the ends of a Sleeping Beautyelement. How would you go about obtaining mice or zebra fish that express the gfpgene?
Step by Step Answer:
Principles of Genetics
ISBN: 978-1119142287
7th edition
Authors: D. Peter Snustad, Michael J. Simmons