Question
A 10-5 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated
A 10-5 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 35 colonies of bacteria grow on the plate.
Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed
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Dilution 10 5 Volume of inoculum taken form 10 5 dilution 010 mL No of CFU arising from 010 inoculum ...Get Instant Access to Expert-Tailored Solutions
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