0 . Examine the results of a microdilution assay, which was performed just like the tube macrodilution testing you did in lab but on a much smaller scale. The concentration of antibiotic was serially diluted in each column from left ( 1 - most concentrated) to right ( 10 - least concentrated) as indicated by the numbers below. The last two columns represent the experimental controls for growth and sterility, respectfully. Shaded wells represent turbidity, or microbial growth, following proper incubation. Each row (AH) corresponds to a different species of bacteria that was tested for antibiotic sensitivity. g/mL antibic a. (5 pts) For which organisms/rows (AH) can a MIC be conclusively determined from these results? b. (2 pts) Name the organism/row (A-H) which had the lowest MIC. What is the MIC for that organism? c. (5 pts) What would you do in order to determine an MBC for organism/row A? Be specific in the steps you would take and how you would make the determination of MBC in your answer. d. (5 pts) If you were to perform a Kirby-Bauer disk diffusion assay with this same antibiotic at a concentration of 4g/mL, which organisms from the plate (AH) would you expect to have zones of inhibition? (Exclude the organisms for which a MIC could not be determined in this experiment.)