1. [20 pts] In the following enzymatic reaction scheme (1) where E, S, ES, EAC, and P represent an enzyme, a substrate, a noncovalent enzyme-substrate complex, a covalent enzyme-substrate complex, and a product, respectively, ki ka k3 S+E ES EAc E + P (1) k-1 A ratio of kz/k3 determines how much fraction of enzyme molecules is present in the form of the covalent enzyme-substrate complex (=EAc) for a given [S]. In which range of the ke/k3 value, can all enzyme molecules be assumed to be present in the form of EAC (ie, [EAc)/{[E]+[EAc]+[ES]} - 1) for any value of [S]? Show your work for credits. Apply PSSA on ES and EAC