1. (4 pts.) If a protein has an isoelectric point (pl) of pH5.2 what kind of ion-exchange column would you use to separate it from a protein with a pl of pH8.9 ? What buffer conditions would you use to equilibrate the column and to elute the proteins off of the column? Why? 2. (4 pts.) Name 4 uses of a gradient maker a) b) c) d) 3. (4 pts.). A particular gene is 1.8Kbp in length. There are two copies of the gene in every diploid cell. A human being has about 1013 diploid cells. How many g of this gene are present in a human being? (Hint: how many copies in total in a human?)