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1. [a] Consider a culture of C. acetobutylicum which produces a potential biofuel in a chemostat operated at steady state. The liquid feed is sterile

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1. [a] Consider a culture of C. acetobutylicum which produces a potential biofuel in a chemostat operated at steady state. The liquid feed is sterile and contains 50 mM com syrup as its only carbon source. In a series of steady state runs, the following results in Table Q.1 [a] were obtained. Table Q.1 [a] D (h-2) 0.06 0.12 0.18 0.24 0.30 0.36 0.42 0.48 0.50 0.52 X (g/L) 1.84 2.32 2.58 2.74 2.84 2.86 2.83 2.55 2.32 1.42 [i] What does this data show? Using these data discuss how would you operate the chemostat to optimize the production of the biofuel? Explain the basis for your answer. [12 marks] [ii] If C. acetobutylicum can also produce antibiotics, what will be your suggestion to optimize the production? Justify your answer. [8 marks]

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