1. Below is a mature cukaryotic mRNA transcript. Translate this mRNA into a protein, also showing the tRNA anticodons involved. Make sure you start and end translation in the right place! Label the ends of the polypeptide chain as N and C terminus. mRNA: 5'GMUUACAUGCGGCUCAGUUGAGGCGAAAAAA 3' tRNA: amino acids:

3. For each of the following mutations, describe the expected effect on the final protein (how will it be different from the protein without a mutation? How likely is it to be functional?) HINT: consider the role of the various sequences in translation! a. An insertion of 3 nucleotides within the coding region b. A deletion of 5 nucleotides within the coding region c. A substitution of one base-pair within the Kozak seqpence (changing GC to AT) d. A substitution of one base-pair within the S'UTR but not affecting the Kozak sequence e. A mutation within the start codon, changing AUG to ACG. f. An insertion of 8 nucleotides within the 3' UTR.