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1. Consider the enzymatic reaction E+Sk1ESESk2E+SESk3EPEPk4P+E Here, E represents an enzyme site, S is the reactant, P is the product, and EX is species X

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1. Consider the enzymatic reaction E+Sk1ESESk2E+SESk3EPEPk4P+E Here, E represents an enzyme site, S is the reactant, P is the product, and EX is species X bound to the enzyme site. We consider the species bound to an enzyme site as reactive intermediates. a) Apply the pseudo steady state hypothesis (PSSH) to ES and solve for [ES] in terms of [E] and [S]. b) Apply the PSSH to EP and solve for [EP] in terms of [E] and [S]. c) Show the enzyme site balance. Leave in terms of [E] and [S] (do not try to manipulate the algebra to solve for [E]). d) What is the rate of generation of product P in terms of [E] and [S]

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