1. The binding affinity of hemoglobin (Hb) for O2 decreases as pH decreases (referred to as the Bohr Effect). Early investigators performed O2 binding studies in a bifunctional buffer system containing cacodylatic acid (pKa = 6.3) and Tris (pKa = 8.1). Over what pH range is this Tris-cacodylate buffer effective? At the low end of the pH range, what is the ratio of cacodylate/cacodylic acid? At the high pH end, what is the ratio of Tris base/TrisHCl?