$1.$ Transfer Buffer $($make $2$ L batches to fill $16$ L carboy$)$

$0\mathrm{.}025$ M Tris pH $8\mathrm{.}3\_\_\_\_\_\_$ g

$0\mathrm{.}192$ M Glycine $\_\_\_\_\_\_$ g

$20\%$ v$\backslash$v Methanol $\_\_\_\_\_\_$ mL

dH$2$O $-->2$L

$2.$ Destain

$40\%($v$/$v$)$ Methanol $\_\_\_\_\_\_\_$ mL

$10\%($v$/$v$)$ Acetic Acid $\_\_\_\_\_\_\_$ mL

dH$2$O $-->500$ mL

$3.0\mathrm{.}25$ M Sucrose $\_\_\_\_\_\_\_\_\_\_\_\_$g$/250$ mL

$4.70\%$ Ethanol $($v$/$v$)\_\_\_\_\_\_\_\_\_\_\_\_\_$mL ethanol$/100$ mL

$5.10\%$ SDS $($m$/$v$)----\_\_\_\_\_\_\_\_\_$ g$/50$mL

Tris $121\mathrm{.}14$ g$/$mole

Sucrose $342\mathrm{.}29$ g$/$mole

Glycine $75\mathrm{.}07$ g$/$mole