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1) Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. a) Calculate the tyrosinase activity (U/mL) by
1) Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. a) Calculate the tyrosinase activity (U/mL) by using the experimental data given below: E = 3600 Mcm2 (molar absorption coefficient for the product dopachrome) v = 0.004 L (final volume in the cuvette) A at 475 nm 0.1091 time (min) 0 0.5 1 1.5 2 2.5 3 10.219 0.3186 0.4106 0.4956 0.5724 0.6451 b) If the tyrosinase concentration in the medium is 0.2 mg/ml, find the specific enzyme activity
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