1) What is the function of Acrylamide/bis in the SDS-PAGE gel? (I point) 2) What is the function of SDS in SDS-PAGE? (1 point) To break the disulphide bonds of proteins disnpting the tertiary structure of proteins along with some reducing agents. 3) What is the function of dithiothreitol (DTT) or 2-mercaptoethanol (beta mercaptoethanol (BME)? (1 point) 4) After the mixing with SDS-sample buffer, the protein is (positively or negatively) charged and migrates from the end to the end across the gel. (1.5 point) 5) The longer the protein is, the (slower or faster) the protein will migrate and have a (longer or shorter) travel distance. (1 point) 6) Why do you need stacking gel locating upper to resolving gel? (1 point) 7) Please plot a calibration curve of log(MW) ( y-axis) versus distance travelled ( x-axis) using the following data and determine the slope. -(1.5 point) 8) Using the calibration curve above, what is the molecular weight of a protein that moved 7.5cm ? kg/mol (1.5 point) 9) Using the calibration curve above could you determine the molecular weight of a protein that moved 12cm ? (YES or NO) ( 0.5 point)