1. What is the purpose of using immersion oil with the 100x objective? 2. You are examining a prepared slide with the 40X objective in place over the microscope. You rotate the 100X objective over the slide. When you look into the eyepiece, your specimen is gone! What happened? 3. What is the difference between magnification and resolution? Review question lab 2: 1. Define the following terms: a. pure culture b. sterile medium c. inoculum d. aseptic techniue e. Colony. 2. State and define the three types of growth that may be seen in a broth culture. a. b. . 3. Define the following terms: a. Chromogenic b. water-soluble pigment c. Water-insoluble pigment. Review Questions lab3 1. What are the two purposes of heat fixation? a. b. 2. What is the purpose of simple staining? 3. Name three basic stains. a. b. C.