Question
1. Which of the following cannot be used for the separation of nucleic acids? a) SDS - PAGE b) PAGE c) Northern blotting d) PAGE
1. Which of the following cannot be used for the separation of nucleic acids?
a) SDS - PAGE
b) PAGE
c) Northern blotting
d) PAGE
2. The polymerization of the gel used in PAGE occurs between polyacrylamide and ____________
a) N, N - acrylamide
b) Bisacrylamide
c) N - methyleneacrylamide
d) N, N - methylene bisacrylamide
3. If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis?
a) 3
b) 4
c) 5
d) 6
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4. The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA?
a) Stacked between histone molecules
b) Binds to the nucleotide base
c) Intercalated between the stacked bases
d) Binds to the phosphodiester backbone
5. Pulse field gel electrophoresis separates DNA molecules of size ___________
a) 10 - 20 bp
b) 20 - 30 Kb
c) 30 - 50 Kb
d) 40 - 50 bp
6. Which of the following will migrate faster? The condition is the molecular weight of the following is equal.
a) Supercoiled circular DNA
b) Nicked circular DNA
c) Single stranded DNA
d) Double stranded DNA
7. Agarose can be extracted from which of the following?
a) Gracilaria esculentus
b) Lycazusican esculentum
c) Ficum benghalensis
d) Agrostis stolonifera
8. Electrophoresis cannot be used to separate _______________
a) DNA
b) RNA
c) Amino acid
d) Protein
9. Which of the following is not a character of polyacrylamide gel?
a) Inert
b) Ionic strength
c) Stable over a wide range of pH
d) Separate upto a few 100 bp of DNA
10. Pulse field gel electrophoresis was developed by ____________
a) Collins and John
b) Kary Mullis
c) Patrick O' Farrell
d) Schwartz and Cantor
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