1. Which of the following is an important step in the preparation of a proper bacterial smear? A. Label the underside of the slide before transferring the microbe. B. Allow the loop to cool slightly after it has been flamed. C. Apply methanol to the smear. D. Make the smear is as thick as possible. E. Add heat to the slide to dry the smear. 2. When transferring cells from an agar or broth, it is necessary to sterilize your inoculating loop to avoid cross- contamination. When should the loop be heat sterilized with the Bunsen burner? A. Do not use the heat with the inoculating loop. B. After the smear and contact with the cell. C. Before picking up the cells from the culture media. D. Before and after contact with the cells. E. Never apply heat to the inoculating loop. 3. When transferring an inoculum from a solid medium, it is necessary to resuspend the culture to help avoid cell clumping. In what substance should the cells be resuspended? A. water B. ethanol. C. immersion oil D. E. crystal violet broth from the culture 4. Sometimes bacterial cultures suspended in broth culture sediment to the bottom of the tube. To resuspend the culture, you should A. invert the tube several times gently tap the tube B. C. shake the tube D. mix the culture with a vortex E. stir the culture with the inoculating loop.