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1) Why do we use phosphoramidites, especially those with diisopropylamine groups, during chemical synthesis of DNA? 2)Sanger sequencing relies on one particular analytical instrument to

1) Why do we use phosphoramidites, especially those with diisopropylamine groups, during chemical synthesis of DNA?

2)Sanger sequencing relies on one particular analytical instrument to achieve single-nucleotide-resolution separation. What is the name of the instrument?

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