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136. To cleave pBR322 at the rop site which restriction enzyme is used? (1) PvuI (3) Pst II (2) Pvu II (4) Hind III

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136. To cleave pBR322 at the rop site which restriction enzyme is used? (1) PvuI (3) Pst II (2) Pvu II (4) Hind III 137. Biolistics is a method of? (1) Artificial gene synthesis (2) Identification of recombinant host (3) Making recombinant DNA (4) Direct gene transfer in host cell 138. Trichoderma, baculovirus and dragon flies are:- (1) Plant pests (2) Used as biocontrol agents (3) Broad spectrum larvicides (4) Source of antibiotics 139. Find out the correct match from the following table:- Column I Column II Column III a-B (1-2) Hydrolysis 1 Maltose linkage starch 2 Cellulose 3 Lactose a- (1-4) and a (1-6) linkage Stored in liver B (1-4) linkage Milk (1) 2 only (2) 2, 3 only (3) 3 only (4) 1, 2, 3 only

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Here are the correct answers along with explanations 136 The correct answer is 1 Pvu I The pBR322 plasmid contains a rop gene that encodes the replication control protein ROP To cleave pBR322 specific... blur-text-image

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