15 pts. Genome annotation vii. Use PROKKA and DFAST to annotate the best Listeria monocytogenes assembly (without the contaminants). Use F23 as the locus_tag prefix and autoincrement values by 10. For PROKKA, enforce GenBank compliance. viii. Compare the total number of proteins predicted by PROKKA and DFAST for your assembly. Are the results congruent? Explain your answer. 15 pts. Contamination removal (from sequencing datasets) ix. Let's filter out the sequencing datasets to keep only the reads from Listeria monocytogenes. Use your best assembly as reference. We can use get_SNPs.pl (--rmo + --bam), minimap2 and samtools bam2fq for this purpose. GZIP your FASTQ outputs. Compare the number of reads before/after with FASTQC. Compare the *.fastq.gz sizes with du -sh too