1)Explain why emission wavelengths are always at higher wavelengths (lower energy) than excitation wavelengths (higher energy). See Jablonski diagram to explain. Note: max excitation is not the light source from the spectrometer.

2) Why are the two LED light sources (blue and green) located at 90o to the detector, while the white light source is located directly in-line with the detector?

3) Is the SpectroVis spectrometer capable of providing excitation spectra, emission spectra or both? Explain.

4)Why was the emission spectrum for fluorescein using the green LED so much larger than the emission using the blue LED?

5)Why was the emission for the quinine almost non-existent for both blue and green LED light sources?