1-What is the difference between ESI-MS spectrum and CID spectrum of leuenkephalin?a. Protonated ions are seen in ESI-MS but not in CID spectrum. b. Doubly protonated ions form.  c. Fragmentation products of leuenkephalin is seen in ESI-MS but not in CID spectrum.  d. Fragmentation products of leuenkephalin is seen in CID but not in ESI-MS. e.Protonated ions are seen in CID spectrum but not in ESI-MS spectrum

2-According to the determination of N,N-dimethylaniline in penicillin by GC, which of the following is not correct?

I. N, N-dimethylaniline is used during the purification of penicillin.  II. N, N-dimethylaniline is an impurity in penicillin.  III.N, N-dimethylaniline cannot be removed from penicillin  IV. Maximum amount of N, N-dimethylaniline allowed is 20 ppm according to pharmacopoeia  V.Naphthalene is used as an internal standard for the measurement of penicillin by GC. VI. Sample and standard solution of N, N dimethylaniline are analyzed and the peaks obtained are compared. VII. For the analysis of N, N-dimethylaniline in penicillin, N, N-dimethylaniline is dissolved in NaOH and extracted to cyclohexane.

a. II, V, VI   b. III, V, VII  c. I, III, VII    d. II, III, V  e. III, IV, VII

3-in quantitation of penicillin by UV Vis spectroscopy, which one of the following is correct? I.Imidazole is a derivatization reagent.  II.Penicillin does not have a chromophore. III.Penicillin derivatize has a maximum absorbance somewhere between 325 and 345 nm

a. I and III  b. Only II  c. I, II and III  d. I and II  e. II and III

4-Which one of the following may act as interference in fluorescence emission spectroscopy?

I. Heavy ions  II.Complex formation  III. UV absorbing species

a. Only III b. II and III  c. I, II and III   d. I and III  e. I and II

1-What is the difference between ESI-MS spectrum and CID spectrum of leuenkephalin?a. Protonated ions are seen in ESI-MS but not in CID spectrum. b. Doubly protonated ions form.  c. Fragmentation products of leuenkephalin is seen in ESI-MS but not in CID spectrum.  d. Fragmentation products of leuenkephalin is seen in CID but not in ESI-MS. e.Protonated ions are seen in CID spectrum but not in ESI-MS spectrum

2-According to the determination of N,N-dimethylaniline in penicillin by GC, which of the following is not correct?

I. N, N-dimethylaniline is used during the purification of penicillin.  II. N, N-dimethylaniline is an impurity in penicillin.  III.N, N-dimethylaniline cannot be removed from penicillin  IV. Maximum amount of N, N-dimethylaniline allowed is 20 ppm according to pharmacopoeia  V.Naphthalene is used as an internal standard for the measurement of penicillin by GC. VI. Sample and standard solution of N, N dimethylaniline are analyzed and the peaks obtained are compared. VII. For the analysis of N, N-dimethylaniline in penicillin, N, N-dimethylaniline is dissolved in NaOH and extracted to cyclohexane.

a. II, V, VI   b. III, V, VII  c. I, III, VII    d. II, III, V  e. III, IV, VII

3-in quantitation of penicillin by UV Vis spectroscopy, which one of the following is correct? I.Imidazole is a derivatization reagent.  II.Penicillin does not have a chromophore. III.Penicillin derivatize has a maximum absorbance somewhere between 325 and 345 nm

a. I and III  b. Only II  c. I, II and III  d. I and II  e. II and III

4-Which one of the following may act as interference in fluorescence emission spectroscopy?

I. Heavy ions  II.Complex formation  III. UV absorbing species

a. Only III b. II and III  c. I, II and III   d. I and III  e. I and II