2. (10 pts) Pseudomonas sp. bacteria are grown in a 15 L CSTR/chemostat on limiting substrate, acetate. The cells are growing in the exponential growth phase with a mass doubling time of 2.33 hours. The saturation constant (Ks) is 1.65 g/L, and cell yield on acetate is 0.41 g cell/g acetate. If a chemostat is operated with a feed stream containing 38.2 g/L acetate, find the following: , , A. the maximum dilution rate (Dmax) B. the steady state substrate concentration in the CSTR when the dilution rate is 0.5 Dmax, 0.8 Dmax, and 0.9 Dmax C. the steady state cell concentration when the dilution rate is half of the maximum (Dmax), 0.8 Dmax, and 0.9 Dmax D. the rate of cell production DX (gdw cells/L-h) and total cell production rate FX (gdw cells/h) when operated at 0.5 Dmax, 0.8 Dmax, and 0.9 Dmax. E. Calculate Dopt (optimum dilution rate to maximize cell production). Discuss your answers to parts C, D & E. Do they make sense? , 2. (10 pts) Pseudomonas sp. bacteria are grown in a 15 L CSTR/chemostat on limiting substrate, acetate. The cells are growing in the exponential growth phase with a mass doubling time of 2.33 hours. The saturation constant (Ks) is 1.65 g/L, and cell yield on acetate is 0.41 g cell/g acetate. If a chemostat is operated with a feed stream containing 38.2 g/L acetate, find the following: , , A. the maximum dilution rate (Dmax) B. the steady state substrate concentration in the CSTR when the dilution rate is 0.5 Dmax, 0.8 Dmax, and 0.9 Dmax C. the steady state cell concentration when the dilution rate is half of the maximum (Dmax), 0.8 Dmax, and 0.9 Dmax D. the rate of cell production DX (gdw cells/L-h) and total cell production rate FX (gdw cells/h) when operated at 0.5 Dmax, 0.8 Dmax, and 0.9 Dmax. E. Calculate Dopt (optimum dilution rate to maximize cell production). Discuss your answers to parts C, D & E. Do they make sense