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2. (6 points, 10min ) The Jmol page for this question contains structures for the complex between two different Fv fragments (A & B) bound

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2. (6 points, 10min ) The Jmol page for this question contains structures for the complex between two different Fv fragments (A \& B) bound to the same dinitrophenyl ligand (aminoDNP). The chemical structure of this hapten is shown on the right. In answering the following questions, you should focus on the two nitro groups (NO2) as well as the aromatic ring on the DNP, the ethylamine group (CH2CH2NH3+)is not important for binding. i) Give the residue name and number (position in sequence) of the tryptophan residues that contact the hapten, also indicate whether they are on the heavy or light chain (2 pts). Hint: When you place your cursor over an atom the residue and chain are displayed. ii) What are the two energetically favorable interactions between these Trp residues and the hapten that would stabilize its bound state? ( 2 pts) iii) Antibody A binds the hapten more tightly than B, why? [Hint: Are there any additional interactions between the hapten and A versus B?] (2 pts)

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