2. A graduate student has recently identified a soluble factor secreted by dendritic cells that have been stimulated with a TLR ligand. He believes that this factor is a novel cytokine. Please design two experiments, including a cellular and a molecular approach to support his hypothesis. (8 ^) 2. A graduate student has recently identified a soluble factor secreted by dendritic cells that have been stimulated with a TLR ligand. He believes that this factor is a novel cytokine. Please design two experiments, including a cellular and a molecular approach to support his hypothesis. (8 ^)