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2. Formation of C and D from reactants A and B occurs as per the mechanism ( - catalytically active site) (1) A+k2k1A,r1 (2) Ak3E+C,r2

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2. Formation of C and D from reactants A and B occurs as per the mechanism ( - catalytically active site) (1) A+k2k1A,r1 (2) Ak3E+C,r2 (3) E+Bk5k4EB,r3 (4) EBk6D+,r4 where E is an active site that still contains a molecular fragment of A left behind after release of C. Invoke PSSH for different forms of enzyme active sites. Derive rate expressions for consumption of A and B and production of C and D, obtain relations among these, and observe their consistency with the reaction stoichiometry A+BC+D Sketch variation in the overall reaction rate r with variations in CA ( CB fixed) and CB ( CA fixed)

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